cellenCHIP 384-3'RNA Seq Kit
Miniaturized library preparation starting from single cells
cellenCHIP 384-3’RNA-Seq Kit enables highly sensitive transcriptomic analyses (3’scRNA-Seq) from individual cells in nanoliter volume using the cellenONE® single cell isolation and dispensing
The combination of cellenONE® and the cellenCHIP 384-3’RNA-Seq Kit enables:
- Analysis of 96 up to 1536 single cells per run
- Handling of diverse sample types: including cell lines, primary cells, rare cells CTC, cell subtypes, dissociated fresh or frozen tissue and nuclei
- Use of a wide range of cell sizes and shapes
The kit is designed to create high quality single cell 3´transcriptome libraries by efficiently capturing poly-adenylated mRNA from single cells: contains 4 cellenCHIP 384s RTready and all reagents to produce ready to sequence libraries for 96 to up to 1536 single cells per run
- cellenCHIPs 384 RTready are preloaded with unique oligo-dT-Primer comprising a cell and molecular barcode, and a combined Lysis Buffer and RTready mix.
- provided with 4 different sets of lllumina compatible library indices allowing multiplexing of up to 16 libraries (6144 cells)
cellenCHIP 384-3’RNA-Seq Kit facilitates pilot studies and generate preliminary data with reduced upfront costs and lower costs per sample
Efficient & streamlined workflow for transcriptomic profiling of biological systems
Benefits of the cellenCHIP
Best in class – Global performance surpassing current gold standard
- >7000 genes detected with only 150,000 reads per single cell
Convenient & simplified handling
- Everything provided to go from cells to ready to sequence libraries, incl. Illumina compatible indices
Adaptable to any samples
- Suited for samples from <10 to 1000’s cells of different size in a perfect controlled manner
Powerful protocol and chemistry
- Optimal workflow – 100 cells sufficient to analyze the heterogeneity of cells
Applications
cellenCHIP 384-3’RNA-Seq Kit allows library preparation from picogram input amounts of RNA for use in stem cell, immuno-oncology or cancer research
- Study CTCs or other liquid biopsy material
- Analyze cancer stem cell heterogeneity
- Identify tumor cell clone’s resistance to therapy
- Investigate complex signaling pathways
- Profile tumor cells and tumor-infiltrating immune cells
cellenCHIP 384 Product Line
cellenCHIP 384
cellenCHIP 384 Accessory Kit
COMING SOON
Related Downloads
Application Note
Characterization of HepaRG® cells grown in 2D or 3D culture at single cell level using the cellenCHIP 384-3’RNA-Seq Kit
Poster
cellenONE®: an innovative tool for high quality and sensitivity 3’ scRNA-seq libraries in nanoliter volume
