cellenCHIP 384-3’RNA Seq Kit

Miniaturized library preparation starting from single cells

cellenCHIP 384-3’RNA-Seq Kit enables highly sensitive transcriptomic analyses (3’scRNA-Seq) from individual cells in nanoliter volume using the cellenONE® single cell isolation and dispensing

The combination of cellenONE® and the cellenCHIP 384-3’RNA-Seq Kit enables:

Analysis of 96 up to 1536 single cells per run
Handling of diverse sample types: including cell lines, primary cells, rare cells CTC, astrocytes and glial cells, cell subtypes, dissociated fresh or frozen tissue and nuclei
Use of a wide range of cell sizes (0,5 µm-100 µm) and shapes
The kit is designed to create high quality single cell 3´transcriptome libraries by efficiently capturing poly-adenylated mRNA from single cells: contains 4 cellenCHIP 384s RTready and all reagents to produce ready to sequence libraries for 96 to up to 1536 single cells per run

cellenCHIPs 384 RTready are preloaded with unique oligo-dT-Primer comprising a cell and molecular barcode, and a combined Lysis Buffer and RTready mix.
provided with 4 different sets of lllumina compatible library indices allowing multiplexing of up to 16 libraries (6144 cells)
cellenCHIP libraries (index tagmentation) are efficiently indexed in the first steps of the library preparation and hence show less carryover
Index hopping rates as low as 0.01% eliminate the need for unique dual-indexing.

cellenCHIP 384-3’RNA-Seq Kit facilitates pilot studies and generate preliminary data with reduced upfront costs and lower costs per sample

Efficient & streamlined workflow for transcriptomic profiling of biological systems

Benefits of the cellenCHIP

Best in class – Global performance surpassing current gold standard

7000 genes detected with only 150,000 reads per single cell

Convenient & simplified handling

Everything provided to go from cells to ready to sequence libraries, incl. Illumina compatible indices

Adaptable to any samples

Suited for samples from <10 to 1000’s cells of different size in a perfect controlled manner

Powerful protocol and chemistry

Optimal workflow – 100 cells sufficient to analyze the heterogeneity of cells
Get morphological and fluorescence info linked to transcriptomic profile of each cell

Applications

cellenCHIP 384-3’RNA-Seq Kit allows library preparation from picogram input amounts of RNA for use in stem cell, immuno-oncology cancer research, neurology and cardiology.

Study CTCs or other liquid biopsy material
Analyze cancer stem cell heterogeneity
Identify tumor cell clone’s resistance to therapy
Investigate complex signaling pathways
Profile tumor cells and tumor-infiltrating immune cells
Study the rare CEC’s or the large cardiomyocytes isolated with the cellenONE
Support brain research by investigation expression profiles of astrocytes, microglial cell and oligodendrocytes.

cellenCHIP 384 Product Line

cellenCHIP 384

cellenCHIP 384 Accessory Kit

Related Downloads

Application Note

Characterization of HepaRG® cells grown in 2D or 3D culture at single cell level using the cellenCHIP 384-3’RNA-Seq Kit

Poster

cellenONE®: an innovative tool for high quality and sensitivity 3’ scRNA-seq libraries in nanoliter volume

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