cellenCHIP 384-3'RNA Seq Kit
Miniaturized library preparation starting from single cells
cellenCHIP 384-3’RNA-Seq Kit enables highly sensitive transcriptomic analyses (3’scRNA-Seq) from individual cells in nanoliter volume using the cellenONE® single cell isolation and dispensing
The combination of cellenONE® and the cellenCHIP 384-3’RNA-Seq Kit enables:
- Analysis of 96 up to 1536 single cells per run
- Handling of diverse sample types: including cell lines, primary cells, rare cells CTC, astrocytes and glial cells, cell subtypes, dissociated fresh or frozen tissue and nuclei
- Use of a wide range of cell sizes (0,5 µm-100 µm) and shapes
The kit is designed to create high quality single cell 3´transcriptome libraries by efficiently capturing poly-adenylated mRNA from single cells: contains 4 cellenCHIP 384s RTready and all reagents to produce ready to sequence libraries for 96 to up to 1536 single cells per run
- cellenCHIPs 384 RTready are preloaded with unique oligo-dT-Primer comprising a cell and molecular barcode, and a combined Lysis Buffer and RTready mix.
- provided with 4 different sets of lllumina compatible library indices allowing multiplexing of up to 16 libraries (6144 cells)
- cellenCHIP libraries (index tagmentation) are efficiently indexed in the first steps of the library preparation and hence show less carryover
- Index hopping rates as low as 0.01% eliminate the need for unique dual-indexing.
cellenCHIP 384-3’RNA-Seq Kit facilitates pilot studies and generate preliminary data with reduced upfront costs and lower costs per sample
Efficient & streamlined workflow for transcriptomic profiling of biological systems
Benefits of the cellenCHIP
Best in class – Global performance surpassing current gold standard
- >7000 genes detected with only 150,000 reads per single cell
Convenient & simplified handling
- Everything provided to go from cells to ready to sequence libraries, incl. Illumina compatible indices
Adaptable to any samples
- Suited for samples from <10 to 1000’s cells of different size in a perfect controlled manner
Powerful protocol and chemistry
- Optimal workflow – 100 cells sufficient to analyze the heterogeneity of cells
- Get morphological and fluorescence info linked to transcriptomic profile of each cell
Applications
cellenCHIP 384-3’RNA-Seq Kit allows library preparation from picogram input amounts of RNA for use in stem cell, immuno-oncology cancer research, neurology and cardiology.
- Study CTCs or other liquid biopsy material
- Analyze cancer stem cell heterogeneity
- Identify tumor cell clone’s resistance to therapy
- Investigate complex signaling pathways
- Profile tumor cells and tumor-infiltrating immune cells
- Study the rare CEC’s or the large cardiomyocytes isolated with the cellenONE
- Support brain research by investigation expression profiles of astrocytes, microglial cell and oligodendrocytes.
cellenCHIP 384 Product Line
cellenCHIP 384
cellenCHIP 384 Accessory Kit
COMING SOON
Related Downloads
Application Note
Characterization of HepaRG® cells grown in 2D or 3D culture at single cell level using the cellenCHIP 384-3’RNA-Seq Kit
Poster
cellenONE®: an innovative tool for high quality and sensitivity 3’ scRNA-seq libraries in nanoliter volume
