proteoCHIP LF 48

The first solution for end-to-end miniaturized single cell proteomics sample preparation for label-free MS-based analysis


proteoCHIP LF 48 is an innovative solution for label-free single cell proteomics sample preparation. With its optimal design and accessories, it further extends the capabilities of cellenONE® single cell isolation technology. The combination of both technologies enables all the front-end of the mass spectrometry including incubation steps.

The proteoCHIP LF 48 allows novel miniaturized single cell proteomics applications in nanoliter-scale volumes on a microscope slide footprint. Each proteoCHIP allows processing of up to 48 single cells, and up to 3 chips can be run at once on cellenONE®, resulting in the preparation of up to 144 single cells per run.

The chips can then be directly interfaced with the LC-MS/MS system or manually transferred into relevant containers (e.g. Evotips).

Benefits of proteoCHIP LF 48

  • Standardization: automated dispensing instead of manual pipetting enables more reproducibility
  • Miniaturization: nanoliter reaction volumes increase sensitivity
  • Throughput: prepare up to 144 single cells per run on cellenON
  • No vial exchanges, direct connection to the autosampler
  • Data quality: identify and quantify >1000 proteins per single cell
  • Clustering and differentiation of cell types

proteoCHIP LF 48 Workflow

Ready to unleash the potential of single cell proteomics?

proteoCHIP LF 48 runs on

  • Image-based single cell selection and isolation (morphology & fluorescence)
  • Precision reagent dispensing (pL – nL) for sample preparation
  • Environmental controls for on-deck incubation