Cell Line Development

Gentle single cell sorting & isolation for cloning applications, with outstanding cell viability and unprecedented clonal outgrowth

Challenges of cell line development

The main limitations associated with monoclonal cell line development include maintaining cell viability and ensuring monoclonality of isolated cells. Unlike cell sorters that induce a lot of stress on cells and thus affect viabilities, automated cloning using cellenONE® provides unseen clonal outgrowth with most cell lines.

Applications

Gene editing (CRISPR/CAS9)

Monoclonal antibodies (mAb production)

Stem cells

Cloning

The best platform for monoclonal cell line development

Researchers are looking for the best technologies and solutions to isolate single cells. At Cellenion, we have developed cellenONE, the most accurate and flexible single cell platform.

Isolate any cell or nuclei (0.5 – 80 µm), including bacteria and other microbial cells, rare cells, cardiomyocytes, etc.)

Up to 100% single cells in account of image-based cell selection

Isolate 96 single cells in < 3 min

Select only cells of interest (morphology & multichannel fluorescence)

Unparalleled clonal outgrowth achieved in 96, 384 and 1536 Microtiter Plates

Unparalleled clonal outgrowth in 96 384 and 1536 MTP

cellenONE® is an ideal platform for undertaking automated cloning in both standard (96 MTP) and high density (384 and 1536 MTP) substrates as it ensures monoclonality while maintaining outstanding clonal outgrowth.

Compatibility with high-density substrates offers a dramatic increase in throughput to automatically process the equivalent of 16 individual 96 MTP in under an hour, leaving personnel free to perform fewer routine tasks.

In addition to saving time, one can save on consumables and expensive reagents while ensuring documentation and monoclonality of all isolated cells.

Assurance Of Monoclonality

For most cell line development applications, single cell images recorded by the cellenONE® will be sufficient to prove monoclonality.

To fullfill certain regulatory processes, monoclonality verifications post isolation might be required. In those cases, cellenONE® can easily be combined with monoclonality verification imagers to provide the required documentation and reports.

In a study, the Celigo® imager from Nexcelom was seamlessly combined with the cellenONE®. Both systems use brightfield and multi-fluorescence cell imaging and are compatible with a wide range of 96, 384, and 1536wp, making these perfect partners for rapid cell line development.

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Cell Line Development

Challenges of cell line development

Applications

Gene editing (CRISPR/CAS9)

Monoclonal antibodies (mAb production)

Stem cells

Cloning

The best platform for monoclonal cell line development

Unparalleled clonal outgrowth achieved in 96, 384 and 1536 Microtiter Plates

Unparalleled clonal outgrowth in 96 384 and 1536 MTP

Assurance Of Monoclonality

Related Products

cellenONE X1 Neo®

cellenONE® HT

cellenONE® X1 BSC

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