Single Microbial
Cell Isolation
Access the microbial individual level
Single Microbial
Cell Isolation
Access the microbial individual level
The most significant discoveries in microbiology were made thanks to culture-based methods, metagenomics and metatranscriptomics studies, but while the former give a truncated and artificial vision of natural reality, the latter give an averaged information, rarely disentangled. Daily, microbiology theories and hypotheses are rising, creating the need for breakthrough innovations, allowing us to access the microbial individual level such as single individual microbial cells, genomes, and transcriptomes. Single microbial cell studies, using cellenONE®, will open unprecedented perspectives in all fields of microbiology.
Applications
Post-Metagenomics and -Metatranscriptomics Microbiota Analyses using cellenONE® Technology
µ-Bio
So far the cellenONE® has been successfully used for isolation of
cellenONE® offers a great alternative to tedious clone picking approaches for single bacteria cloning.
A range of bacteria have been successfully isolated on cellenONE® systems using both IBSCI™ and FIBSCI™ technologies.
For smaller cells, including bacteria, the use of fluorescent labels in conjunction with FIBSCI™ is recommended.
List of bacterial genera successfully isolated so far:
- Bacillus
- Escherichia
- Lactobacillus
- Microccocus
- Pseudomonas
- Staphylococcus
Filamentous fungal spores of around 2 to 3.5 µm, as well Saccharomyces cerevisiae (also known as baker´s yeast), with sizes ranging from 3 to 9 µm, were successfully isolated with the cellenONE® X1 systems using IBSCI™
List of fungi successfully isolated so far:
- Aspergillus Niger
- Aspergillus Oryzae
- Saccharomyces cerevisiae
Single microalgae and diatoms “with sizes from 9 to 48 µm” were successfully isolated for scMS with the cellenONE® X1 system using IBSCI™:
- Alexandrium Minutum
- Thalasiossira Weissflogii
- Lauderia sp.
Pseudomonas fluorescens with Deep Red and Syto13, 40x magnification
