Single Cell Omics

Scalable nanoliter volume library preparation of single cells

Applications

Most current methods are based on either microfluidics platforms, which do not offer multiple interventions for complex sequencing libraries preparations, or on large volume microplate-based sample preparations that tend to be expensive due to reagent costs.

cellenONE® technology breaks away from these limitations by combining the small volumes found only in microfluidics with the flexibility of a liquid handler to enable scalable nanoliter volume library preparation of single cells.

scRNA-Seq

scWGS

scATAC-Seq

Miniaturized 3' Scrna-seq

A 3’ scRNA-seq protocol was developed in the cellenCHIP using a range of commercially available reagents. The following steps describe the workflow using cellenCHIP and cellenONE®

Single Cell Transcriptiomics

Open Platform

Transcriptiomics Success Story

Marco Marra’s lab and collaborators

(University of British Columbia, BC Cancer Vancouver)

First miniaturised Total scRNAseq method (lysis in 5.5 nL!)
First stranded Total scRNAseq method
First automatized Total scRNAseq method

“We demonstrate that our method […] enabled the profiling of transcript regions beyond only transcript termini, and yielded data rich enough to allow identification of cell types from heterogeneous biological samples”

Haile et al. 2021, Frontiers in Genetics

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Single Cell Omics

Applications

cellenONE® technology breaks away from these limitations by combining the small volumes found only in microfluidics with the flexibility of a liquid handler to enable scalable nanoliter volume library preparation of single cells.

scRNA-Seq

scWGS

scATAC-Seq

Miniaturized 3' Scrna-seq

Single Cell Transcriptiomics

Open Platform

Transcriptiomics Success Story

“We demonstrate that our method […] enabled the profiling of transcript regions beyond only transcript termini, and yielded data rich enough to allow identification of cell types from heterogeneous biological samples”

Haile et al. 2021, Frontiers in Genetics

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cellenONE® X1 Neo

proteoCHIP Product Line

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