Single Cell Omics
Scalable nanoliter volume library preparation of single cells
Single-Cell Omics
Scalable nanoliter volume library preparation of single cells
Most current methods are based on either microfluidics platforms, which do not offer multiple interventions for complex sequencing libraries preparations, or on large volume microplate-based sample preparations that tend to be expensive due to reagent costs.
cellenONE® technology breaks away from these limitations by combining the small volumes found only in microfluidics with the flexibility of a liquid handler to enable scalable nanoliter volume library preparation of single cells.
Applications
scRNA-Seq
scWGS
scATAC-Seq
Miniaturized 3' scRNA-seq
A 3’ scRNA-seq protocol was developed in the cellenCHIP using a range of commercially available reagents. The following steps describe the workflow using cellenCHIP and cellenONE®
Single Cell Transcriptiomics
Open Platform
- Any single-cell omics approaches (genomics, epigenetics, transcriptomics, proteomics, metabolomics…)
- Compatible with most commercially available and published protocols
- Just dispense single cells in any dedicated reagent x consumable
Transcriptiomics Success Story
Marco Marra’s lab and collaborators
(University of British Columbia, BC Cancer Vancouver)
- First miniaturised Total scRNAseq method (lysis in 5.5 nL!)
- First stranded Total scRNAseq method
- First automatized Total scRNAseq method
