Thank you for taking the time to fill out the CELLENION Newsletter Sign up. Your email will be included in our next campaign.

cellenONE F1.4

cellenONE® F1 is our latest development, based on both IBSCITM and  FIBSCITM  technologies, it uses cellenONE® X1 platform but now allows detection and imaging of fluorescent markers excited by up to 4 different light sources.


  • Technical Specifications

    4- fluorescent excitation channels

    2 microplate holders compatible with 96, 384, 1536wp, and microwell substrates

    Up to 2 nanoliter dispensing channels (only one is used for single cell isolation)

    Temperature control from -20°C to 120°C

    Humidity and dew point control as an option

cellenONE F1.4


cellenONE F1.4
Channel colours Excitation Wavelength (nm) Emission Wavelength (nm)
Blue 380 410 - 450
Green 475 500 - 530
Orange 555 575 - 615
Red 635 660 - 800

Unique Features


Single Cell Sequencing
Open platform Choose your substrate (96, 384, 1536wp or microwells)
Versatile Suitable for a range of sequencing methods
Adaptable Isolate cells, nuclei (from FF or FFPE tissues), microbeads
Optional Use a second dispensing channel for nanoliter dispensing of reagents (barcodes, lysis buffers…)

Cell Line Development
Gentle Acoustic technology ensures outstanding cell viability
Efficient Only single cell are dispensed, cell line development time can be slashed by several weeks
Optimal Use Live/Dead kit to isolate exclusively live single clones

Suitable Applications



Remove dead cells from sequencing libraries
Isolate live cells only
Isolate one subpopulation
Isolate best expressers
Enhance clonal recovery
Allow multiple fluorescent marker visualisation
Multichannel sorting possible (+/– selections)
Isolate very rare cells from complex samples
Live/Dead assays

  • Successfully isolated cells and particles

    Cell lines CHO, hybridoma, HEK293T, HeLa, A549, PC3, H1975, HepaRG, Jurkat, 3T3, SKBR3, PC9, THP-1
    Primary cells PBMC (including B- and T- cell fractions), fibroblasts, keratinocytes, melanocytes, cardiomyocytes, HUVEC, neural stem cells
    Nuclei from cell line, fresh tissues, fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue slices
    Microorganisms Bacteria, Spores, Microalgae & Diatoms, Pollen, Yeast
    Microbeads PMMA, PS, hydrogel microbeads from 2 to 50µm diameters, TOYOPEARL®, 10x Genomics Gel Beads
  • Successfully tested fluorescent dyes

    Excitation ChannelFluorescent Dyes
    BlueDAPI, Hoechst 33342, Violet Dead Cell Stain, ELF® 97
    GreenFITC, GFP, AlexaFluorTM 488, CellTrackerTM Green CMFDA, CFSE, Acridine Orange
    OrangePE, AlexaFluorTM 568, AlexaFluorTM 555, Cy3, mCherry, CellTrackerTM Orange CM-Dil, Propidium Iodide
    RedAlexaFluorTM 647, Cy5, CellTrackerTM Deep Red



Compare our products

 cellenONE® X1cellenONE® F1.4
Product NumberF00CF00CF4
Single Cell Isolation Technology


Image-Based Single Cell Isolation


Both Non-Fluorescent and Fluorescent Image-Based Single Cell Isolation

cellenONE Control Module

Monitor cells and particles: its position and geometrical properties inside the PDC

Isolation of most cells with diameters ranging from 5 to 60 µm

cellenTILEsOrganise and review images of isolated cell for QC purpose
cellenREPORTGenerate a comprehensive report containing all isolation data
High Resolution Optical based Single Cell Isolation Method6.4M pixels CMOS camera (3088*2076 pixels)3.2M pixels CMOS camera
(2056*1542 pixels)
Fluorescence ExcitationNoneFour Colors
Visualisation of fluorescently labelled particles





BenefitsHeterogeneous samples and unlabelled particlesSorting sub-populations
Rare cells isolation