Researchers at the Genome Sciences Center in British Columbia (Canada) have developed an automatized method of sample preparation for DNA sequencing.
Using cellenONE technology, single cells are isolated in an open nano-well structure; the chip is populated with various reagents and cells allowing interaction between them. The system is based on scalling down standard library construction reactions by 1000x so the proportion of reagents and nucleic acids remains approximately the same at the single cell scale.
The efficient isolation of single cells is a decisive part to complete the full workflow for single cell whole genome sequencing with no pre-amplification and, separately, single cell whole transcriptome sequencing, each capable of processing up to 3000 cells per run.
