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proteoCHIP EVO 96

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Easy, Loss-Minimized Sample Prep for Single-Cell Proteomics

The proteoCHIP EVO 96 is built for researchers aiming at large-scale, reproducible single-cell proteomics. With 192 wells per chip, it enables preparation of hundreds of single-cell samples in under three hours. Its standout feature is seamless transfer to Evotips by centrifugation, allowing direct interfacing with the Evosep One LC system. This streamlined clean-up and transfer minimizes peptide loss and background noise, delivering high sensitivity and robustness for discovery-driven studies, comparative population screens, and spatial proteomics.

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Benefits

With up to 192 cells per run prepared in under 3 hours, EVO 96 streamlines high-throughput proteomics with minimal hands-on time.

Centrifugation-based transfer to Evotips eliminates unnecessary handling, reducing peptide loss and preserving signal for deeper proteome coverage.

Automated protocols reduce variability between runs and labs, ensuring robust and reproducible results for large-scale studies.

EVO 96 workflows are fully contained in cellenONE, enabling smooth sample prep from single-cell isolation to LC–MS/MS compatibility.

By operating at nanoliter scale, EVO 96 reduces background noise and saves on reagents while enhancing proteome coverage.

Dedicated support and training help researchers quickly implement EVO 96 workflows, ensuring robust results from the start.

“Our workflow sets a benchmark in SCP for sensitivity and throughput, with broad applications in basic biology and biomedicine for identification of cell type-specific markers and therapeutic targets.” Ye, Z., Sabatier, P., van der Hoeven, L. et al. Nature Methods 22, 499–509 (2025)

Validated on
proteoCHIP EVO 96

Key Features
1

adapter for optimal temperature transfer in cellen one

2

Easy single-orientation loading simplifies workflow setup

3

96 wells per chip, up to 192 cells per run

4

Direct fit on Evotip racks for clean transfer: seamless, low-loss loading to Evosep One by centrifugation

5

Fabricated in low-retention material to safeguard peptide recovery

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