cellenONE®

The open platform for single cell isolation and nanoliter dispensing

cellenONE® X1

Image-based single-cell sorting and dispensing in 96, 384 & 1536 well plates or cellenCHIP

cellenONE® F1.4

Multi-fluorescence image-based single-cell sorting and dispensing in 96, 384 & 1536 well plates or cellenCHIP

cellenONE® BSC

Image-based single-cell sorting and dispensing in 96, 384 &1536 well plates or cellenCHIP, integrated under a class II biosafety cabinet

High accuracy single cell isolation and dispensing

cellenONE® is a revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image processing that provides real-time and high accuracy single cell isolation and dispensing.

Using gentle acoustic waves for droplet generation, cellenONE® preserves outstanding cell viability for all cloning applications and maintains protein expressions for sequencing applications.

Versatile technology for a variety of samples

High-resolution optics allows isolation of any cell type (mammalian cells, bacteria, fungi, archea, plants, etc…) from any sample size.

Working in aspirate/dispense mode allows high recovery of a wide range of samples, from minute cell suspensions containing just a couple of microliters and a few dozen cells to much larger samples containing thousands of cells.

Benefits of cellenONE®

  • High accuracy single cell isolation and dispensing

  • Gentle on cells, ensuring outstanding cell viability

  • Compatible with a wide range of particle sizes (0.5 µm – 80 µm)

  • Ideal for rare cell samples (zero dead volume and high recovery)

  • High-throughput isolation of hundreds of cells in only a few minutes

Benefits of cellenONE

  • High accuracy single cell isolation and dispensing

  • Gentle on cells, ensuring outstanding cell viability

  • Compatible with a wide range of particle sizes

  • Ideal for rare cell samples (zero dead volume and high recovery)

  • High-throughput isolation of hundreds of cells in only a few minutes

Applications

cellenONE® are low volume, high viability and high accuracy single cell isolation systems for both single cell omics and cell line development applications.

Single Cell Omics

Cell Line Development

We have worked successfully with a large variety of cell types

CHO, Hybridoma, HEK293T, HeLa, A549, PC3, H1975, HepaRG

From cell line, fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue slices

PMMA microbeads from 2 to 30µm diameters

TOYOPEARL®

PS microbeads

PBMC (including B- and T- cell fractions)

Fibroblasts, keratinocytes, melanocytes, cardiomyocytes, HUVEC

Neural Stem Cells

Application notes

SCoPE2-MS using cellenONE®

Unparalleled clonal outgrowth in 96 384 and 1536 MTP

Scaling Whole Genome Sequencing to > 50,000 Single cells using cellenONE®

Product specifications

 

cellenONE®  X 

cellenONE®  F1.4 

Sorting Technology 

Brightfield images

Brightfield and/or
Multi-fluorescence images
(Blue, Green, Orange and Red)

Dispensing Technology 

sciDROP PICO:  250-600 pL / single drop 

Number of Dispensing Channels 

Up to 2 

Spottable Area (X x Y) 

252 x 112 mm 

Target Capacity 

2 MTPs or 8 standard glass slides  

Axis System 

X-Y Linear Magnetic Drive, Z Spindle Drive

Axis Precision 

< 3 µm 

Dimensions with Enclosure (L x W x H) 

650 x 700 x 1590 mm 

Weight  

205 kg 

cellenONE® X1

For applications where sorting based on cell morphology is sufficient

cellenONE® F1.4

For applications requiring multichannel fluorescence sorting and imaging

cellenONE® BSC

For applications where full protection of sample, operator, and environment is required