Cell Line Engineering
Redefining What’s Possible in Cell Line Engineering
Cell line engineering is transforming the way researchers design, study, and harness living cells. By introducing precise genetic modifications, scientists can reprogram cellular behavior to create models of disease, enhance therapeutic production, or unlock entirely new biological functions. From CRISPR-mediated knock-ins to multi-marker reprogramming, CLE offers unparalleled opportunities to tailor cell lines to specific research or industrial goals.
This application is critical in areas such as regenerative medicine, oncology, and advanced therapy development. Researchers face challenges that include tracking engineered cells with multiple markers, isolating rare populations with high fidelity, and ensuring robust clonal expansion for downstream studies.
At this intersection of complexity and precision, cellenONE® X1 NEO provides unique advantages. With four fluorescence channels for multi-parameter sorting, image-based isolation, and consistently high outgrowth rates, it enables scientists to push the boundaries of cell line engineering while maintaining reproducibility and scalability.
You're in good company
These leading institutions use our single cell and 3D cell model technologies:
Cell Line Engineering applications
Cell Reprogramming
Reprogram cells into new functional states using transcription factors or markers, for disease modeling, regenerative medicine, and personalized therapies.
Gene Editing & Functional Studies
cellenONE X1 NEO supports CRISPR/Cas9 or other editing workflows with precise single-cell isolation and clonal expansion for functional validation of engineered lines.
iPSC ENGINEERING FOR CELL THERAPIES
With cellenONE, generate and optimize induced pluripotent stem cell lines with high outgrowth rates and controlled expansion.
A Precision Tool for Cell Line Engineering
Typical Cell line Engineering workflow
Featured resources
APPLICATION NOTE
Charité hiPSC Subcloning with cellenONE
From the Core Unit for Stem Cells at BIH-Charité, led by Dr. Harald Stachelscheid with Dr. Valeria Fernandez-Vallone, this note details how cellenONE supports subclonal iPSC isolation. Their group enables pluripotent stem cell derivation, genetic manipulation, and quality control, now complemented by image-based subcloning workflows for higher fidelity.
POSTER
Sub-Confluent, Sub-Clonal Workflow for Single-Cell Isolation
A refined workflow that captures cells before reaching full confluency, enabling early isolation of subclonal populations. This resource shows how pre-emptive isolation can enhance reproducibility in CLE experiments.
PUBLICATION
Molecular Therapy: High-throughput Single-Cell Gene Editing
This peer-reviewed article demonstrates how single-cell technologies enable precise gene editing in therapeutic workflows. The findings highlight how image-based selection and high-fidelity isolation technologies complement modern CLE strategies.