Cell Line Development
Cell Line Development: Building Reliable Production Platforms
Cell line development (CLD) is the foundation of modern biopharmaceutical manufacturing. To produce monoclonal antibodies, recombinant proteins, or viral vectors at scale, regulatory authorities require therapeutic cell lines to be derived from a single progenitor cell. This ensures product consistency, genetic stability, and full traceability.
The CLD process spans multiple critical steps, from transfection and selection to cloning, screening, and expansion. Each stage must balance productivity, viability, and regulatory compliance, while timelines often stretch across months of intensive work. One of the most decisive points is single-cell isolation, where robust technologies are needed to ensure monoclonality and secure downstream success.
At Cellenion, we support CLD by providing flexible, modular solutions for single cell isolation. The cellenONE instrument range integrates advanced features such as four fluorescence channels, multi-source to multi-target dispensing, and high outgrowth rates. Our cellenONE HT, designed as an agnostic, automation-ready platform, adapts seamlessly to diverse workflows and connects with other systems across the development pipeline, making it an ideal solution where more complex CLD challenges go beyond the reach of fully automated platforms.
You're in good company
These leading institutions use our single cell and 3D cell model technologies:
Cell Line Development applications
Monoclonal Antibody Production
Establish stable, high-producing clones for antibody manufacturing with proven clonality and consistent productivity.
With cellenONE, researchers can combine precise single-cell isolation, fluorescence-based marker detection, and environmental control to generate clones that thrive. The high outgrowth rate and flexibility to integrate into different downstream platforms make cellenONE HT an ideal choice for robust, compliant therapeutic antibody workflows.
Vaccine Development
Generate clonal cell lines for vaccine antigen production, ensuring reproducibility and scalability across multiple platforms.
Recombinant Protein Manufacturing
Accelerate development of cell lines producing complex proteins, from enzymes to growth factors, with reproducible quality.
Making Cell Line Development Workflows Smarter
Typical Cell line development workflow
Featured resources
WEBINAR
Scaling B-cell Cloning Workflows for Antibody Development
See how cellenONE enables next-generation B-cell cloning workflows with unmatched flexibility. By pairing image-based single-cell selection with precision microdispensing, researchers can scale antibody discovery far beyond traditional limits, ensuring higher throughput, clonality, and reproducibility.
APPLICATION NOTE
Multisample Runs Made Simple
Discover how cellenONE turns complex multisample workflows into streamlined processes. With fully programmable runs and true flexibility in design, the system handles parallel samples effortlessly, accelerating CLD timelines while maintaining precision and compliance standards.
Application Note
Exceptional Clonal Outgrowth at Any Scale
From 96- to 1536-well plates, cellenONE delivers industry-leading clonal outgrowth rates that other systems struggle to achieve. This resource highlights how our unique isolation and dispensing technology ensures robustness at scale, making cellenONE the platform of choice for demanding CLD applications.