sciFLEXARRAYER enables the work we are doing: spotting about one nanoliter scale cells and a few nanoliters for various reagents and a few more nanoliters for tagmentation reaction. The sciFLEXARRAYER is spatially accurate, volumetrically accurate and is able to spot each cell, one after another, into the well. Without the sciFLEXARRAYER and cellenONE we wouldn´t be able to do this work!
We study methods for library preparation for single cells called STRAND-Seq use to study structural variation and ultimately cancer. We got a cellenONE X1 in December (2018) and since then we have been able to dispense single cells and reagents; and essentially make a library preparation protocol working in nanolitre volumes.
We are hoping to increase throughput for library preparation by using nanolitre volumes so we can decrease the amount of reagents we use and then we should be able to essentially mass produced libraries using cellenONE dispensing; to produce many at a time and in a short tie period. We will become more of a manufacturing process.
We were checking and comparing different single cell technologies, and we really think this is the most advanced one because there is a high accuracy for single cells, high viability, as well as an open platform where we can do RNA-seq, DNA-seq and all kind of experiments without buying a kit that is closed. And this quite important for us because we want to measure intra tumour heterogeneity inside tumours.
We were looking for a machine that could dispense single cells or nuclei; that is quite flexible as well and has minimal loss of material while the material is spotted on the microwell plate.
Cellenion team has been quite supportive to get many, many different protocols up and running in our lab, working with very difficult samples, that´s been great support over the months and years.
We can finally have an automated workflow and offer it as well to other researchers at our institute.