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Welcome to the Cellenion Hub for the AACR Virtual Annual Meeting II.
Here we have collated information and resources to provide insight into how the cellenONE® technology can assist with your cancer research and diagnostics.
If you would like to learn more about our technology and discuss your applications, contact us for a free consultation and demo. Don't forget to quote "AACR2020" for your exclusive offer on our cellenONE instruments.
Thank you for visiting, and we look forward to seeing you again in-person at the AACR Annual Meeting in 2021!
cellenONE, an automated single cell dispensing system based on patented piezo-acoustic technology, allows precise cell deposition on a wide range of microplates (96, 384, 1536) and microwell substrates.
Most dispensing and microfluidic technologies follow Poisson distribution, which leads to multiple cells per position and low efficiency. cellenONE uses software-integrated visual feedback to ensure only single cells are deposited in every position.
cellenONE only dispenses drops containing single cells to your preferred substrate. All remaining drops will be dispensed into a recovery tube, resulting in no sample wastage. Additionally, the cellenONE isolates cells utilizing an acoustic technology which has been shown to be gentler than the high stress of FACS and results in excellent cell viability.
cellenONE® is a low volume, high viability and accuracy single cells isolation technology for both sequencing and cell line development applications.
Click the icons below to learn more.
Single Cell Omics
Cell Line Development
Not only does cellenONE provide high accuracy single cell isolation, but also a number of additional features to miniaturize your library preparation:
Benefits of library preparation miniaturization with cellenONE:
These benefits can be amplified when used in conjunction with our newly developed cellenCHIP.
By combining cellenONE’s single cell isolation and nanoliter dispensing features, the groups of Sam Aparicio (UBC, Vancouver) and Sohrab Shah (MSKCC, New York) have developed a scalable single cell whole genome sequencing method called Direct Library Preparation Plus (DLP+).
Benefits of DLP+ include:
This method is now implemented as a routine at the British Columbia Cancer Research center and being adopted by leading research centers worldwide.
Method developed by:
This method is now implemented at these world leading research centres:
The following application notes demonstrate some of the capabilities of the cellenONE technology.
ACCURATE AND PRECISE SPIKE-IN METHOD FOR CTC VALIDATION STUDIES WITH CELLENONE®
Abstract
Analysis of circulating tumor cells (CTCs) from a routine blood draw has great potential for cancer diagnostic and treatment monitoring applications. The rarity of CTCs, often present at a frequency of 1 CTC per 10 billion red blood cells, demand technologies such as RareCyte’s AccuCyte® - CyteFinder® system which combines exquisite sensitivity and specificity with a reproducible end-to-end workflow.
RareCyte develops tools and assays for CTC enumeration and biomarker expression analysis for breast, prostate, and lung cancers. As diagnostics and clinical trials require accurate, precise, and reproducible CTC detection, rigorous assay validation is essential. For analytical validation of enumeration accuracy, it is critical to generate surrogate blood samples with known numbers of model CTCs (mCTCs) at levels near the limit of detection for the assay. These studies require an accurate and precise number of spike-in samples performed in a reproducible manner which is not possible by either serial dilution or flow cytometry approaches.
Developed by Cellenion, the cellenONE® instrument is a single-cell isolation and dispensing technology using automated image recognition to accurately ensure that each dispensed droplet contains only one cell. While the cellenONE is primarily used for single cell analyses and cell line development, Cellenion and RareCyte have collaborated to develop a new application of this technology. Here we demonstrate how RareCyte employed the cellenONE instrument to generate samples required for CTC assay validation. The cellenONE instrument proved essential to accuracy studies and is now the system of choice for mCTC sample preparation for these studies.
cellenONE® X1 supports single cell isolation by visualization and dispensing a range of cells and particles from 2 to 70 um diameter based on image-based (IBSCITM) software.
It offers a fully automated and integrated system with high speed and precision robotics.
Cellenion has developed cellenCHIP™, an innovative nanowell chip offering miniaturized wells to enable open reactions in nanoliter volumes. To successfully miniaturize library preparation, it is essential to use substrates that have been designed for sub-microliter volume work.
Each cellenCHIP contains 4 x 96 quadrants resulting in 384 wells on a microscope slide footprint, and up to 8 cellenCHIPs (3072 single cells) can be placed on a cellenONE deck.
Convert your microliter library preparations to highly efficient nanoliter reactions with dramatic cost savings.
We were looking for a machine that could dispense single cells or nuclei; that is quite flexible as well and has minimal loss of material while the material is spotted on the microwell plate.
Cellenion team has been quite supportive to get many, many different protocols up and running in our lab, working with very difficult samples, that´s been great support over the months and years.
We can finally have an automated workflow and offer it as well to other researchers at our institute.
cellenONE: An Open Platform for Single Cell Isolation and Miniaturizing Library Preparation
Presented by Guilhem Tourniaire, Scientific and Managing Director, Cellenion
Optimising Circulating Tumour Cells Isolation for scRNA-Seq Analyses
Presented by Guilhem Tourniaire, Scientific and Managing Director, Cellenion
Recent Advances in Mass Spectrometry of Small Mass Samples is Enabling Single Cell Proteomics
Presented by Nathan Manes, PhD, National Institute of Allergy and Infectious Diseases, NIH
Exceptional single cell outgrowth meets indisputable full well imaging validation
Automated single cell dispensing and enhanced imaging to accelerate your cell line development.
Presented by:
Cellenion was founded in 2016 in Lyon, France as a fully owned company of SCIENION, the global leader in precise, low volume dispensing and detection applications in life sciences and diagnostics.
The cellenONE utilizes SCIENION’s technologies and specializes in development of controlled cell isolation and printing applications.
Cellenion’s team has extensive know-how in application support for cell and biomaterial dispensing and offers services to enable your ideas from R&D through to scale-up.
We would love to discuss your applications and see if the cellenONE can support your efforts further.
Our team of experts are on-hand to provide an obligation-free consultation and demo.
Phone: +33 986 48 70 70
Email: [email protected]
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