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[centered_container hex=”#666666″][wc_headings header=”The proteoCHIP 12*16 is an innovative solution enabling multiplexed single cell mass spectrometry-based proteomics analysis. It enables all the front-end of the mass spectrometry on cellenONE, including incubation steps.” size=”h2″ textalign=”center”][/centered_container]
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The proteoCHIP 12*16 has a microscope slide footprint, allowing novel miniaturized single cell proteomics applications in nanoliter-scale volumes.
Each proteoCHIP contains 12 arrays (pools) of 16 wells each, enabling peptide labelling with TMTpro (either 11-plex or 16-plex). The cellenONE can handle up to 3 chips per run.
After labelling, the cells can be easily pooled with the proteoCHIP Funnel. A dedicated Accessory Kit provides all the adapters to facilitate the use of proteoCHIP and Funnels with cellenONE, centrifuge, and LC-MS/MS autosampler.
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Benefits of proteoCHIP 12*16
- Standardization: automated dispensing instead of manual pipetting enables more reproducibility
- Miniaturization: nanoliter reaction volumes save reagents costs and increase sensitivity
- Throughput: prepare up to 576 single cells per run on cellenONE
- Process safety: unique design and material prevents cross-contamination, while prefilling with oil overcomes evaporation
- Recovery: direct injection via autosampler leads to maximum liquid recovery and very little peptide loss
- Data quality: detect and quantify up to 2000 proteins per single cell, with outstanding signal-to-noise ratio (even with low or no carrier)
