1. Cell suspension is loaded in a glass capillary
2. Capillary tip is placed in front of an optical system
3. Capillary tip is segmented into two zones
Ejection zone: volume corresponding to the next generated droplet
Sedimentation zone: safety zone considering possible cell sedimentation
4. If the next droplet contains only a single cell that fits user-defined parameters (size, shape, fluorescence markers), it is dispensed into target labware. Otherwise, it is dispensed into a recovery vial allowing reprocessing.