cellenONE® X1

Gentle & precise image-based single cell sorting

Applications

Tailored for

  • Single cell omics (any omics, omni- omics)
  • Cell line development (any cell, any standard or custom labware)
  • Precision low-volume dispensing (pL – nL range) for miniaturized sample preparation

Benefits of cellenONE® X1

  • Direct visual inspection of the sample
  • Processing of any sample (from 1 µL and just few cells)
  • Image-based single cell selection (brightfield and/or 4-channel fluorescence)
  • Full image record of isolated cells/clones
  • Best-in-class monoclonal outgrowth rate
  • Environmental controls (temperature, humidity)
  • Up to 100% single cells

How it works

1. Cell suspension is loaded in a glass capillary

2. Capillary tip is placed in front of an optical system

3. Capillary tip is segmented into two zones

Ejection zone: volume corresponding to the next generated droplet
Sedimentation zone: safety zone considering possible cell sedimentation

4. If the next droplet contains only a single cell that fits user-defined parameters (size, shape, fluorescence markers), it is dispensed into target labware. Otherwise, it is dispensed into a recovery vial allowing reprocessing. 

Special feature

Post-isolation monoclonality verification by the operator

Technical specifications

 

 

cellenONE®  X1

Sorting Technology

 

Brightfield and/or
Multi-fluorescence images
(Blue, Green, Orange and Red)

Dispensing Technology

sciDROP PICO:  150-600 pL / single drop

Number of Dispensing Channels

Up to 2

Spottable Area (X x Y)

252 x 112 mm

Target Capacity

2 MTPs or 8 standard glass slides

Axis System

X-Y Linear Magnetic Drive, Z Spindle Drive

Axis Precision

< 3 µm

Dimensions with Enclosure (L x W x H)

650 x 700 x 1590 mm

Weight 

205 kg

 

Discover other products in the cellenONE® line

cellenONE® HT

For full automation of single cell workflows

cellenONE® X1 BSC

For single cell workflows requiring full protection of sample, operator, and environment