cellenONE® X1
Gentle & precise image-based single cell sorting
Applications
CRISPR/CAS9
mAb
Stem cells
scRNA-seq
scWGS
scATAC-seq
scMS
Cloning
Tailored for
- Single cell omics (any omics, omni- omics)
- Cell line development (any cell, any standard or custom labware)
- Precision low-volume dispensing (pL – nL range) for miniaturized sample preparation
Benefits of cellenONE® X1
- Direct visual inspection of the sample
- Processing of any sample (from 1 µL and just few cells)
- Image-based single cell selection (brightfield and/or 4-channel fluorescence)
- Full image record of isolated cells/clones
- Best-in-class monoclonal outgrowth rate
- Environmental controls (temperature, humidity)
- Up to 100% single cells
How it works
1. Cell suspension is loaded in a glass capillary
2. Capillary tip is placed in front of an optical system
3. Capillary tip is segmented into two zones
Ejection zone: volume corresponding to the next generated droplet
Sedimentation zone: safety zone considering possible cell sedimentation
4. If the next droplet contains only a single cell that fits user-defined parameters (size, shape, fluorescence markers), it is dispensed into target labware. Otherwise, it is dispensed into a recovery vial allowing reprocessing.
Special feature
Post-isolation monoclonality verification by the operator
Technical specifications
|
cellenONE® X1 | |
Sorting Technology
|
Brightfield and/or DAPI (ex.375nm; em. 432nm); FITC (ex.490nm; em.515nm); Cy3 (ex.565nm; em.580nm); Cy5 (ex. 625nm; em.670nm) |
|
Dispensing Technology |
sciDROP PICO: 150-600 pL / single drop |
|
Number of Dispensing Channels |
Up to 2 |
|
Spottable Area (X x Y) |
252 x 112 mm |
|
Target Capacity |
2 MTPs or 8 standard glass slides |
|
Axis System |
X-Y Linear Magnetic Drive, Z Spindle Drive |
|
Axis Precision |
< 3 µm |
|
Dimensions with Enclosure (L x W x H) |
650 x 700 x 1590 mm |
|
Weight |
242 kg |