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cellenONE® X1 is our first single cell isolation and dispensing platform. It offers a fully automated and integrated system with high speed and precision robotics.
Based on IBSCITM, it allows visualization and isolation of a range of cells and particles (from 2 to 70µm diameter).
2 microplate holders compatible with 96, 384, 1536wp, and microwell substrates
Up to 2 nanoliter dispensing channels (only one is used for single cell isolation)
Temperature control from -20°C to 120°C
Humidity and dew point control
|Open platform||Choose your substrate (96, 384, 1536wp or microwells)|
|Versatile||Suitable for a range of sequencing methods|
|Adaptable||Isolate cells, nuclei (from FF or FFPE tissues), microbeads|
|Optional||Use a second dispensing channel for nanoliter dispensing of reagents (barcodes, lysis buffers…)|
|Gentle||Acoustic technology ensures outstanding cell viability|
|Efficient||Only single cell are dispensed, cell line development time can be slashed by several weeks|
|Sterile||For fully sterile processing now comes integrated into BSC environment|
|Cell lines||CHO, hybridoma, HEK293T, HeLa, A549, PC3, H1975, HepaRG, Jurkat, 3T3, SKBR3, PC9, THP-1|
|Primary cells||PBMC (including B- and T- cell fractions), fibroblasts, keratinocytes, melanocytes, cardiomyocytes, HUVEC, neural stem cells|
|Nuclei||from cell line, fresh tissues, fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue slices|
|Microorganisms||Bacteria*, Spores, Microalgae & Diatoms, Pollen, Yeast|
|Microbeads||PMMA, PS, hydrogel microbeads from 2 to 50µm diameters, TOYOPEARL®, 10x Genomics Gel Beads|
Suitable for most cell types
Unlike other technologies (microdroplet, microfluidic traps), doublets can be completely excluded and only single cells are dispensed.
Reduce consumable consumption
Outstanding clonal recoveries were observed with 96% , 94% and 94% of wells containing single colonies in 96, 384 and 1536 MTP, respectively.
Additionally, the remaining wells were empty and did not contain multiple colonies.
|cellenONE® X1||cellenONE® F1.1||cellenONE® F1.4|
|Single Cell Isolation Technology|
Image-Based Single Cell Isolation
IBSCI™ and FIBSCI™
Both Non-Fluorescent and Fluorescent Image-Based Single Cell Isolation
|cellenONE Control Module|
Monitor cells and particles: its position and geometrical properties inside the PDC
Isolation of most cells with diameters ranging from 5 to 60 µm
|cellenTILEs||Organise and review images of isolated cell for QC purpose|
|cellenREPORT||Generate a comprehensive report containing all isolation data|
|High Resolution Optical based Single Cell Isolation Method||6.4M pixels CMOS camera (3088*2076 pixels)||3.2M pixels CMOS camera|
|Fluorescence Detection||None||One Color||Four Colors|
|Visualisation of fluorescently labelled particles||Green|
|Benefits||Heterogeneous samples and unlabelled particles||Exclusion of dead cells|
Selection of best expressers
Rare cells isolation