By combining cellenONE’s single cell isolation and nanoliter dispensing features, the groups of Sam Aparicio (UBC, Vancouver) and Sohrab Shah (MSKCC, New York) have developed a scalable single cell whole genome sequencing method called Direct Library Preparation Plus (DLP+).
Results generated have been compiled into a web-based interactive visualisation platform (www.cellmine.org).
This affordable, low bias method allows in depth analyses of a variety of samples.
It allows determination of copy number variation (CNV), single nucleotide polymorphysim (SNP), cluster analyses and phylogenic reconstructions.
This method is now implemented as a routine at the British Columbia Cancer Research center and being adopted by leading research centers worldwide.
Method developed by
High quality single cell whole genome libraries
CNV, SNP, Cluster analyses and Lineage reconstruction
Any cells and nuclei from 5 to 80μm
Low reagent costs (0,27 US$/cell) (ref: BioRxiv)
This method is now implemented at these world leading research centres