Bacteria isolation

 

cellenONE offers a great alternative to tedious clone picking approaches for single bacteria cloning.

A range of bacteria have been successfully isolated on cellenONE systems using both IBSCI and FIBSCI technologies.

For smaller bacteria, the use of fluorescent labels in conjunction with FIBSCI is recommended.

List of bacterial genus successfully isolated so far:

  • Bacillus
  • Escherichia
  • Lactobacillus
  • Microccocus

 

 

Example of colonies grown from single bacteria (E. coli and S. epidermidis) dispensed on agar as a checkerboard pattern. Image courtesy of the Zengler lab, UCSD.

Due to their very small size, bacteria are too small to be seen by IBSCITM only. Fluorescent labels helped successful isolation of various bacteria using FIBSCITM.

 

Micrococcus labelled with CellTracker Deep Red
E. coli labelled with CellTracker Deep Red